FastQCFastQC Report
Thu 16 Apr 2020
FCHHWFYBBXX_L3_CHKPEI85217070035_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameFCHHWFYBBXX_L3_CHKPEI85217070035_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81119211
Sequences flagged as poor quality0
Sequence length49
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG5857100.7220361154646833No Hit
CACACACACACACACACACACACACACACACACACACACACACACACAC3854300.47514022294916053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCGA178950.013.61062343
TATACTG718750.012.0697045
CTATACT641150.011.9806984
GTATTAT231500.011.7235221
TCTATAC140500.011.5493073
CTGTTAA543600.011.486661
ACGCTGC319550.011.48306142
TAACAGT798750.011.4250034
GTATTAG146350.011.3914421
CCTATAC573900.011.2911033
GTATAGA148500.011.1972041
TGACGCT392300.011.10156242
CGCTGCC337950.010.96641443
GTATTAA219250.010.959031
GTATAGG482900.010.947451
GTCATAG202200.010.8820981
ATACTGC642050.010.7800176
GTGTAGG238300.010.7314011
GTTAAAC574250.010.7153333
TTATACT210050.010.6618424
Produced by FastQC (version 0.11.5)